Diafiltration is a dilution process that involves removal or separation of components (permeable molecules like salts, small proteins, solvents etc.,) of a solution based on their molecular size …

Diafiltration is a technique that uses ultrafiltration membranes to completely remove or lower the concentration of salt or solvent, or to replace buffer salts from solutions containing proteins …

Diafiltration is used to exchange the buffer surrounding a molecule by gradually replacing the retentate with a new solution. This allows researchers to keep biologics stable during the …

Diafiltration is a process for separation and purification of the target product out of the main solution containing the other small molecular weight (MW) substances, e.g. salts, solvent. The …

Diafiltration is a technique that uses ultrafiltration membranes for the separation of sugars, salts and acids from solutions containing proteins, pectin and other macromolecules …

Diafiltration or DF (sometimes referred to as buffer exchange) involves the addition of buffer such that the liquid surrounding the target is exchanged to one that is preferred for storage stability.

Diafiltration operates on the fundamental principle of selective permeation through a semipermeable membrane, which allows smaller molecules to pass through while retaining …

Learn diafiltration techniques for desalting and buffer exchange of biological samples. Explore continuous & discontinuous methods.

Ultrafiltration & diafiltration are important steps throughout bioprocessing to increase capacity, concentration, and recovery. Increasingly, single-pass tangential flow filtration (SPTFF) is …

The molecular weight cut-off (MWCO) chosen for diafiltration should be at most half the estimated molecular weight of the smallest protein intended for analysis.