E.coli Expression Vectors: Cloned Gene Under Control of Regulated Promoter: λPL,T7, pBAD, pET vector
27:51
YouTubeRohini Patil
E.coli Expression Vectors: Cloned Gene Under Control of Regulated Promoter: λPL,T7, pBAD, pET vector
The overexpression of genes has deleterious effect on foreign gene cloned in E.coli host. Thus avoid this controlled promoters are used like λ PL, T7, pBAD. All these expression systems are discussed. You may contact rohini.coaching@gmail.com
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Brianna Bibel on Instagram: "Does the plasmid you ordered have 2 antibiotics listed? One might actually be for cells that plasmid is in (e.g. BL21(DE3)-RIL) which themselves have a plasmid, containing genes for tRNAs for rare codons… https://youtu.be/uUFuyu0gQ9k BL21 is the workhorse E. coli strain for recombinant protein expression. Extra things can be added and/or the bacterial genome can be modified to help optimize the cells for making various proteins. For example, you can add in extra copi
9:38
Brianna Bibel on Instagram: "Does the plasmid you ordered have 2 antibiotics listed? One might actually be for cells that plasmid is in (e.g. BL21(DE3)-RIL) which themselves have a plasmid, containing genes for tRNAs for rare codons… https://youtu.be/uUFuyu0gQ9k BL21 is the workhorse E. coli strain for recombinant protein expression. Extra things can be added and/or the bacterial genome can be modified to help optimize the cells for making various proteins. For example, you can add in extra copi
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